Measurement

Part:BBa_K902025:Design

Designed by: Somshukla Chaudhuri   Group: iGEM12_Calgary   (2012-10-02)

Moco Riboswitch- GFP(LVA)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 779


Design Notes

There are no illegal cut sites. Changes to the DNA sequence can inactivate the brick through changes in the RNA secondary structure.

Source

The Moco Riboswitch was synthesized from IDT. The GFP(LVA) part was used from the registry parts. This brick was assembled using the biobrick assembly method.

References

1) Regulski EE, Moy RH, Weinberg Z, Barrick JE, Yao Z, Ruzzo WL, Breaker RR. A widespread riboswitch candidate that controls bacterial genes involved in molybdenum cofactor and tungsten cofactor metabolism.Mol Microbiol. 2008 May; 68(4): 918–932